可变剪接分析(热点)

Designed by novelbio
 ASD(Alternative splicing detector) is a user-friendly software that aims to recognize and detect differential alternative splicing(AS) exons in two samples of RNA-Seq data. Since AS events always have two obvious feature in RNA-Seq data, which are altered junction reads and altered coverage of AS-exons, the algorithm of this software considers these two factors while calculating a combine P-value to investigate whether a AS event is statistically significant or not.


Click button AddBam to load in the bam format files generated from the mapping software such as tophat. Fill the prefix column and group columns. Bam files come from same technical replicates should with same prefix names, and with same group names if they come form same biological replicates.

 

AccID: the gene Access ID or Symbol, depends on the information provide by GTF file.
Location: Splicing events happened location, canbe enter in IGV to see splicing details.
Treat_JuncInfo: Junction reads number information in treat sample.
Col_JuncInfo: Junction reads number information in control sample.
TreatCoverage: Treatment splicing exon coverage and gene coverage
ColCoverage: Control splicing exon coverage and gene coverage
P-value: The significant of differential splicing event.
SplicingType: Type of this alternative splicing site.
Description: Desciption of this gene, depends on the information provide by GTF file.

 
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